By Fibrotic Regulation, Long Noncoding RNA-ATB Promotes the Occurrence and Development of Scleroderma

Abstract

We used intervention means to investigate the mechanism of LncRNA-ATB involved in the development and progression of Scleroderma by examining the expression differences of LncRNA-ATB between lesional skin of Scleroderma patients and normal skin using a cellular model. Lesional skin and normal skin recruited from five patients with Scleroderma diagnosed in the outpatient clinic were designed as experimental and control groups respectively, established a fibroblast cell model. (1) Magnetic nanoparticles aided in the quantitative determination of LncRNA-ATB in tissue and fibroblasts respectively; (2) Determine the level of transcription and protein expression of fibrosis-related genes before and after Scleromderma Group interference. LncRNA-ATB regulates fibrotic mechanisms in Scleroderma by influencing collagen and fibronectin transcriptional synthesis in Scleroderma fibroblasts.