Cu(II) Coordination Complex: Anti-Thyroid Carcinoma Activity by Initiating Reactive Oxygen Species Accumulation and Inducing Cell Apoptosis

Abstract

A mixed-donor ligand that shows pyrazole and carboxylic groups and a coordination complex that include Cu(II) has been created in the present study by mixing Hcppp with Cu(NO3)2 ·3H2O in water and DMF mixed solvent. The chemical formula of the compound is [Cu2(cppp)2(Hcppp)2(H2O)2] ·2NO3 ·6H2O. Additionally, Complex 1’s inhibitory influence against the cell proliferation of K1 human thyroid carcinoma cells was explored via exploiting the Cell Counting Kit-8 analysis. Annexin V-FITC/PI double staining analysis was finished to evaluate Complex 1’s mechanism, which induced the death of K1thyroid carcinoma cells. Moreover, the reactive oxygen species (ROS) analysis was implemented to determine the accumulation of ROS in K1 thyroid carcinoma cells.