Propolis-Loaded Chitosan Nanoparticles for the Treatment of Bacterial Diarrhea: In Vitro and In Vivo Evaluation

Author:

Qiao Jie1,Yang Jun2,Jalili Saman3

Affiliation:

1. Department of Pediatrics, 3201 Hospital, Hanzhong, 723000, China

2. Department of Pediatrics, Xi’an Gaoxin Hospital, Xi’an, 710061, China

3. Department of Biomaterials Science and Technology, Isfahan University of Technology, Isfahan, 548987, Iran

Abstract

Diarrhea is a disorder in the gastrointestinal system that is accompanied by cramps, decreased stool’s viscosity, and reduced defecation intervals. In the current study, propolis was loaded into chitosan nanoparticles to develop a potential treatment for gastrointestinal infections. Various In Vitro experiments were performed to characterize the produced nanoparticles. The healing function of propolis-loaded chitosan nanoparticles (ProCHNPs) was evaluated in a rat model of enteropathogenic Escherichia coli-induced diarrhea. In Vitro studies showed that ProCHNPs were not toxic against intestinal epithelial cells and improved their viability. Furthermore, these particles had around 261.49±63.22 nm average particle size and showed significantly higher antibacterial and anti-inflammatory activities than propolis-free chitosan nanoparticles. In Vivo studies showed that ProCHNPs had comparable anti-diarrhea function with norfloxacin as the standard drug. In addition, ProCHNPs increased the number of beneficial bacteria in the intestinal microflora and reduced the level of pro-inflammatory cytokines in the colon tissue. This study suggests potential use of ProCHNPs as an alternative treatment for bacterial diarrhea.

Publisher

American Scientific Publishers

Subject

General Medicine

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