Propolis-Loaded Chitosan Nanoparticles for the Treatment of Bacterial Diarrhea: In Vitro and In Vivo Evaluation

Abstract

Diarrhea is a disorder in the gastrointestinal system that is accompanied by cramps, decreased stool’s viscosity, and reduced defecation intervals. In the current study, propolis was loaded into chitosan nanoparticles to develop a potential treatment for gastrointestinal infections. Various In Vitro experiments were performed to characterize the produced nanoparticles. The healing function of propolis-loaded chitosan nanoparticles (ProCHNPs) was evaluated in a rat model of enteropathogenic Escherichia coli-induced diarrhea. In Vitro studies showed that ProCHNPs were not toxic against intestinal epithelial cells and improved their viability. Furthermore, these particles had around 261.49±63.22 nm average particle size and showed significantly higher antibacterial and anti-inflammatory activities than propolis-free chitosan nanoparticles. In Vivo studies showed that ProCHNPs had comparable anti-diarrhea function with norfloxacin as the standard drug. In addition, ProCHNPs increased the number of beneficial bacteria in the intestinal microflora and reduced the level of pro-inflammatory cytokines in the colon tissue. This study suggests potential use of ProCHNPs as an alternative treatment for bacterial diarrhea.