Moringa oleifera-Loaded Nanocomposite Scaffolds Augment Bone Injury Healing in a Rat Model of Critical Sized Bone Defect: A Potential Treatment Strategy for Nursing Care in Fracture Patients

Abstract

Nursing and medical care for facture patients is challenged by several issues such as unavailability of a suitable bone graft, challenges associated with autologous bone graphing, and rejection of the bone graft. In the current study, Moringa oleifera extract was loaded into chitosan nanoparticles and the resulting delivery system was added into a collagen solution and lyophilized to produce a bioactive bone graft. Various In vitro experiments were performed to characterize the nanocomposite scaffolds and their healing function was evaluated in a rat model of calvarial defect. In vitro studies showed that the scaffolds protected MG-63 cells against oxidative stress and had a porous microstructure. Histopathological studies showed that the scaffolds loaded with Moringa oleifera extract augmented bone injury healing to a higher extent than other groups. Furthermore, gene expression studies showed that the rats treated with Moringa oleifera extract-loaded scaffolds had significantly higher tissue expression levels of osteopontin, Osteonectin, collagen type 1, collagen type 2, and VEGFa genes.