Healing Efficacy of Hesperetin-Containing Chitosan Gel in Burn Wound: Formulation Design and In Vivo Evaluation

Abstract

More than 5 million people require medical attention due to burn-related injuries annually. Significant research has been carried out in recent decades to develop approaches to improve the healing of burn wounds. The focus has also been on the development of natural product-based therapeutic remedies for the treatment of burn wounds. This has been done primarily due to multimodal mechanisms exhibited by some promising bioactive molecules of natural origin. Hesperetin is one such molecule that possesses strong anti-inflammatory and antioxidant properties. It is mainly obtained from citrus species. The goal of the current study was to assess how well chitosan gel that contains hesperetin may cure burn wounds. The advantage of using chitosan gel is that it could form a depot at the site and provide a protective therapeutic covering over burn wounds. In the present study, hesperetin-containing chitosan gel was prepared and evaluated for percentage hesperetin content, extrudability, spreadability, and rheological behavior. The preclinical wound healing activity was evaluated using an experimental burn wound model in Wistar rats. The results of the animal experiment showed early and better healing of burn wounds in animals treated with hesperetin-containing chitosan gel. There was 92.79% healing after 14 days of application of hesperetin-containing chitosan gel compared to 69.49% healing observed in the control group. Further, the histopathological evaluation suggested no inflammatory cell infiltration, normal epidermal growth, and normal collagen bundle arrangement in these animals. Overall the results provide proof of concept to establish the wound healing potential of hesperetin-containing chitosan gel against burn wounds.