Biosynthetic Luminescent Ag Nanoparticles in Functionalization for Breast Cancer Therapy

Abstract

Breast cancer is still the most common malignant tumor in the world despite the research and technique advances. However, how to come true targeted controlled release perplexing the clinicians all the time and rapid development of nanotechnology, as well as emerging microRNA (miRNA), offers an opportunity for diagnosis and treatment of breast cancer. Here, nude mouse model with breast cancer was built and silver nanoparticles (Ag-NPs, ANPs) with near infrared characteristic were biosynthesized. Then, RNA-seq, western blot analysis, and immunofluorescence of the differential expression spectrum of cultured mouse T cells treated by silver nitrate in vitro hinted that biosynthesis of ANPs in cells depended on participation of ferritin and serum albumin (SA). Subsequently, ANPs loaded with miR-200c (miR-200c@ANPs) in vivo and in vitro showed its well property of intelligent controlled release, function of targeted imaging, and potential targeted therapy. The present data indicated that biosynthesized miR-200c@ANPs was equipped with biocompatibility, intelligent controlled release drug, tumor targeting therapy, as well as the visual function. More, this method may be a promising alternative option for diagnosis and treatment of breast cancer.