The Treatment of Human Colon Xenografts Tumor in Mice with Platinum Nanosphere-5-Fluorouracil-Bovine Albumin

Abstract

Because 5-fluorouracil (FLU) has side effects in cancer treatment, the use of FLU in therapeutic activities is limited. To overcome this challenge, the use of nano-platforms for its targeting is f great interest in biomedical fields. For this purpose, to reduce the FLU toxicity and improve the its efficacy, platinum nanospheres (PtNS) with anti-cancer properties were used. After producing PtNS by hydrothermal method and loading FLU and bovine albumin (bAL) (PtNS-FLU-bAL), its physicochemical properties were investigated. After evaluating the drug release capability, the toxicity of PtNS-FLU-bAL on HCT-116 cells was assessed by MTT and flow-cytometry. Also, the effects of the nanospheres on tumor status, liver and kidney tissues were evaluated. The results indicate uniform size of the PtNS-FLU-bAL (79±2.04 nm) with spherical shape, loading of more than 50% of the FLU (in the ratio of 2:1 FLU to PtNS-bAL), optimal release of the FLU from the PtNS-FLU-bAL (83.1% in pH = 6), and the high toxicity of the PtNS-FLU-bAL on HCT-116 cells. Also, the toxicity mechanism indicated more apoptosis induction by increasing the expression of TNF-α, Bax, Fas, and Caspase-3 genes for PtNS-FLU-bAL compared to the free FLU. Moreover, the results showed a higher FLU concentration in cancerous tissue and a 1.5-fold reduction in tumor growth by the PtNS-FLU-bAL compared to the free FLU. Overall, the results show that the PtNS-FLU-bAL can enhance the success of colorectal cancer treatment effectively and safely.