Affiliation:
1. Department of Pathology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China
2. Department of Pathology, Nanjing Medical University, Affiliated Nanjing Maternity and Child Health Care Hospital, Jiangsu, Nanjing 210004, China
3. Department of Oncology, Shaanxi Cancer Hospital, Xian 710061, China
Abstract
Because 5-fluorouracil (FLU) has side effects in cancer treatment, the use of FLU in therapeutic activities is limited. To overcome this challenge, the use of nano-platforms for its targeting is f great interest in biomedical fields. For this purpose, to reduce the FLU toxicity and
improve the its efficacy, platinum nanospheres (PtNS) with anti-cancer properties were used. After producing PtNS by hydrothermal method and loading FLU and bovine albumin (bAL) (PtNS-FLU-bAL), its physicochemical properties were investigated. After evaluating the drug release capability,
the toxicity of PtNS-FLU-bAL on HCT-116 cells was assessed by MTT and flow-cytometry. Also, the effects of the nanospheres on tumor status, liver and kidney tissues were evaluated. The results indicate uniform size of the PtNS-FLU-bAL (79±2.04 nm) with spherical shape, loading of more
than 50% of the FLU (in the ratio of 2:1 FLU to PtNS-bAL), optimal release of the FLU from the PtNS-FLU-bAL (83.1% in pH = 6), and the high toxicity of the PtNS-FLU-bAL on HCT-116 cells. Also, the toxicity mechanism indicated more apoptosis induction by increasing the expression of TNF-α,
Bax, Fas, and Caspase-3 genes for PtNS-FLU-bAL compared to the free FLU. Moreover, the results showed a higher FLU concentration in cancerous tissue and a 1.5-fold reduction in tumor growth by the PtNS-FLU-bAL compared to the free FLU. Overall, the results show that the PtNS-FLU-bAL can enhance
the success of colorectal cancer treatment effectively and safely.
Publisher
American Scientific Publishers
Subject
Pharmaceutical Science,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering
Cited by
1 articles.
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