Research on Orientation of Basic Fibroblast Growth Factor with Magnetic Nanoparticles (MNPs) on Regeneration and Recovery of Rats’ Dampened Skeletal Muscle and Expressed Level of Matrix Metalloproteinase

Abstract

The basic fibroblast growth factor (bFGF) has a special role in improving proliferation and differentiation of fiber cells in growth of muscle. The function of bFGF with magnetic nanoparticles (MNPs) on regeneration and recovery of rats’ dampened skeletal muscle and expression of MMPS were studied in our research. The MNPs packed with bFGF were prepared and 95 experimental rats were selected. These 30 rats were equally divided into control group, model group (self-healing without obstruction after model was established), bFGF group (disposed with bFGF packaged with MNP). The contractility and stress relaxation of rats’ skeletal muscle were observed at the 48th h, 10th, 17th, 24th and 30th days after damage. The remaining 65 rats were divided randomly into control group (5 rats) and experimental group (60 rats intervened with MNPs packaged with bFGF). The groups were randomly divided into 0.5 h, 1 h, 3 h, 6 h, 12 h, 1 d, 2 d, 3 d, 4 d, 7 d, 10 d and 14 d according to different executed time. The levels of bFGF and MMPS were detected by HE staining method and immunohistochemical staining. There was a significant declining tendency of shrinkage stress of muscle in the model, sham-operation, BSA and bFGF groups compared with control group in the second day. The contractility after contusion wound in the regeneration and recovery of rats’ skeletal muscle was effectively alleviated with MNPs packaged with bFGF.