Research on Mechanism of miR-488-5p Carried with Magnetic Carbon Nanotubes in Restraining Lymphatic Metastasis in Cervical Cancer Through Induction of mTOR/P70S6K Signaling Pathway
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Published:2022-12-01
Issue:12
Volume:18
Page:2701-2707
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ISSN:1550-7033
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Container-title:Journal of Biomedical Nanotechnology
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language:en
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Short-container-title:j biomed nanotechnol
Author:
Wang Donghua1,
Liu Xiaoli1,
Gong Shixiong1,
Jia Yijuan1,
Wang Ping1
Affiliation:
1. Department of Gynaecology and Obstetrics, First Hospital Wuhan, Hubei Province, 430022, China
Abstract
This study intends to discuss mechanism of miR-488-5p carried with magnetic carbon nanotubes (MAGCNTs) in restraining lymphatic metastasis in cervical cancer through induction of mTOR/P70S6K signaling pathway. Fifty female rats were randomly divided into five sets, which included control
set, miR-488-5p set, empty carrier set, set of miR-488-5p carrier and inhibitor set. The MAGCNTs were prepared and characterized. Condition and quantity of lymphatic metastasis in cervical cancer, miR-488-5p expression and protein expression of factors related with mTOR/P70S6K signaling transduction
pathway were observed. Quantity of lymphatic metastasis in the control set was highest, while quantity of lymphatic metastasis in the set of miR-488-5p and carrier were lowest. Expressions of miR-488-5p in of the miR-488-5p and carrier sets were highest and lowest in the control set, and mTOR/P70S6K
expression was reversed. miR-488-5p was carried with multi-walled carbon nanotubes (MWCNTs) and entered into cells more rapidly. There was over expression of miR-488-5p and activity of mTOR/P70S6K signaling pathway was restrained. The information conduction was slowed down, and therefore,
the lymphatic metastasis in the cervical cancer was restrained.
Publisher
American Scientific Publishers
Subject
Pharmaceutical Science,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering