Affiliation:
1. Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Insititute, Shenyang, Liaoning, 110000, China
Abstract
This study explored miR-20a’s role in intestinal cancer cells. SW480 cell line was divided into control group, agonist group, inhibitor group, and negative control group, followed by analysis of cell proliferation, apoptosis, Met, Bad, and Bcl-2 protein expressions. Results showed
that miR-20a expression in the agonist group was the highest, followed by blank group and negative control group, and inhibitor group was lowest. S-phase and G2/M cell number from inhibitor group was lowest, and cell apoptosis rate was highest. However, the agonist group showed contrary changes.
There was no difference in G0/G1 phase cell number among the four groups (P > 0.05). Moreover, the expressions of Bad, Bcl-2, Met, Wnt, β-catenin, and p-Wnt in inhibitor group were all lower, while the expressions in agonist group were all high (P < 0.05). S-phase
and G2/M cell number in inhibitor group was lowest, while cell apoptosis was highest. The agonist group was opposite, with G0/G1 phase cells in each group showing no difference (P > 0.05). Wnt, β-catenin, and p-Wnt expressions were lowest in the inhibitor group, while
the agonist group was opposite. These results together showed that the miR-20a directly targeted and regulated Met protein. Finally, the miR-20a inhibited intestinal cancer cell proliferation mainly through inhibiting Wnt/β-catenin signaling activity.
Publisher
American Scientific Publishers
Subject
Pharmaceutical Science,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering