Nano-Albumin Particles Loaded with miR-20a Inhibitor Targeting Met Protein to Reverse Proliferation of Intestinal Cancer Cells

Abstract

This study explored miR-20a’s role in intestinal cancer cells. SW480 cell line was divided into control group, agonist group, inhibitor group, and negative control group, followed by analysis of cell proliferation, apoptosis, Met, Bad, and Bcl-2 protein expressions. Results showed that miR-20a expression in the agonist group was the highest, followed by blank group and negative control group, and inhibitor group was lowest. S-phase and G2/M cell number from inhibitor group was lowest, and cell apoptosis rate was highest. However, the agonist group showed contrary changes. There was no difference in G0/G1 phase cell number among the four groups (P > 0.05). Moreover, the expressions of Bad, Bcl-2, Met, Wnt, β-catenin, and p-Wnt in inhibitor group were all lower, while the expressions in agonist group were all high (P < 0.05). S-phase and G2/M cell number in inhibitor group was lowest, while cell apoptosis was highest. The agonist group was opposite, with G0/G1 phase cells in each group showing no difference (P > 0.05). Wnt, β-catenin, and p-Wnt expressions were lowest in the inhibitor group, while the agonist group was opposite. These results together showed that the miR-20a directly targeted and regulated Met protein. Finally, the miR-20a inhibited intestinal cancer cell proliferation mainly through inhibiting Wnt/β-catenin signaling activity.