Regulation of Vimentin on Cell Metabolic Signaling Pathway in Muscle Injury

Abstract

The regulation of vimentin on cellular metabolic signaling pathway in muscle injury was analyzed experimentally. Seventy experimental mice were divided into six experimental groups and one control group according to exercise training time. Muscle injury model was established by repeated running and jumping. Vimentin expression was quantified by image analysis method, combined with pathological and ultrastructural changes of muscle tissue, and serum was detected. The regulation of vimentin metabolic signaling pathway in muscle injury cells was comprehensively analyzed by creatine kinase, cell proliferation and vimentin expression time course in skeletal muscle of mice. The results showed that the average optical density and area of vimentin positive area of mice in each experimental group were different with different exercise degrees. During the first 6–7 weeks of training, muscle injury had a process of gradual aggravation and alleviation, which indicated that the muscles showed good adaptability while the injury accumulated. The time course changes showed that there was some expression of vimentin in mice of different experimental groups after exercise. The experimental results confirmed that vimentin had a regulatory role in the metabolic signaling pathway of muscle injury cells.