Competitive Adsorption on Nanomaterials for Human Papillomavirus 16 L1 Protein Sensitive Detection
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Published:2020-12-01
Issue:12
Volume:12
Page:1392-1398
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ISSN:1941-4900
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Container-title:Nanoscience and Nanotechnology Letters
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language:en
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Short-container-title:nanosci nanotechnol lett
Author:
Zhu Li1,
Zhao Yu1,
Yao Shangchen1,
Xu Mingzhe1,
Yin Lihui1
Affiliation:
1. National Medical Products Administration (NMPA) Key Laboratory for Quality Research and Evaluation of Chemical Drugs, National Institutes for Food and Drug Control, Beijing 102629, PR China
Abstract
Human papillomavirus (HPV) L1 protein is a biomarker of HPV infection. The identification of HPV L1 protein provides valuable information regarding the viral infection status, reflect the status of HPV virus replication in cervical cells, and help understand cervical lesions Fade and
progress. Graphene oxide is an important nanomaterial. Owing to the optical properties of graphene oxide (GO) itself and the interaction between GO and HPV16 L1 nucleic acid aptamer, we established a method for quantitative detection of HPV16 L1 protein by UV absorption spectrophotometry.
The recovery rate of the method is 87%–102%, and the limit of detection is 2 pg/mL. We analyzed the HPV16 L1 protein quantitatively in clinical samples by adopting this method. The current method is simple and has promising reliability and profound sensitivity.
Publisher
American Scientific Publishers
Subject
General Materials Science