Role of Long Non-Coding RNA Zinc Finger E-Box Binding Homeobox 1 Antisense 1 in Regulating the microRNAs-200b/Wnt1 Axis Effects on Proliferation, Invasion, and Apoptosis of Gastric Carcinoma Cells
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Published:2020-04-01
Issue:4
Volume:12
Page:556-562
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ISSN:1941-4900
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Container-title:Nanoscience and Nanotechnology Letters
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language:en
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Short-container-title:nanosci nanotechnol lett
Author:
Mi Chen,Ren Li,Zhang Dan,He Shuixiang
Abstract
ZEB1-AS1 is an Long non-coding RNA (lncRNA) that is abnormally expressed in various tumor types and affects tumor etiology and progression. ZEB1-AS1 can affect the malignant progression of gastric cancer through the miRNA-200b/Wnt1 axis. However, its effect on proliferation, invasion,
and apoptosis of gastric cancer is unclear. Using BGC-803 cells, we studied the effects of lncRNA ZEB1-AS1 and miRNA-200b on the proliferation, invasion, and apoptosis of gastric cancer. Using qRT-PCR, we found that the expression of ZEB1-AS1 was significantly increased and the expression
of miRNA-200b was significantly decreased in BGC-803 cells. By CCK-8, Transwell, and flow cytometry assays, we also found that down-regulation of ZEB1-AS1 and upregulation of miRNA-200b inhibited the proliferation and invasion of BGC-803 cells and promoted apoptosis. Furthermore, upregulation
of ZEB1-AS1 and miRNA-200b reversed these effects. LncRNA ZEB1-AS1 inhibits the proliferation and invasion of gastric cancer cells and induces apoptosis by regulating the miRNA-200b/Wnt1 molecular axis.
Publisher
American Scientific Publishers
Subject
General Materials Science