Elevated Expression of miRNA-181a-5p in Placenta Tissue of Preeclampsia

Abstract

This study intends to assess miR-181a-5p expression profile in preeclampsia (PE). PE tissues from 30 patients and 30 normal pregnancy patients were obtained to measure miR-181a-5p level by RTPCR. Cell was transfected with miR-181a-5p mimics followed by analysis of cell migration by woundhealing assay, cell proliferation by CCK-8 assay as well as IGF2BP2 expression by RT-PCR and Bcl-2, Bax, caspase3, and Bcl-xl level by western blot. miR-181a-5p was significantly upregulated in PE patients. Mimics transfection significantly decreased cell migration and proliferation. Interestingly, miR-181a-5p bound to IGF2BP2 3′UTR. Further, miR-181a-5p upregulation decreased IGF2BP2 expression dose-dependently, upregulated the mRNA expression of Bax, caspase3 and Bcl-xl and downregulated Bcl-2. IGF2BP2 upregulated Bcl-2 and downregulated Bax and caspase3, which were reversed by miR-181a-5p. miRNA-181a-5p overexpression restrains placental trophoblast cells by down-regulation of IGF2BP2, thereby participating in PE pathogenesis.