Role of Curculigo Orchioides in the Proliferation and Apoptosis of Breast Cancer Michigan Cancer Foundation-7 (MCF-7) Cells Under Network Pharmacology

Abstract

Based on network pharmacology (NP), the effects of curculigo orchioides (CO) granules alone or CO granules along with tamoxifen on the proliferation and apoptosis (P&A) of human breast cancer (BC) cells were explored. A search database was constructed to obtain the active components (ACs) of CO and related target genes to perform disease mapping, and a BC target map was constructed to perform related pathway enrichment analysis (PEA). The blank control group (CG), estrogen group (EG), and tamoxifen group (TG) were set as controls to observe the effects of CO granules alone and CO granules along with tamoxifen. The PEA showed that the effect of CO on BC may be related to the cooperation of its ACs. The target of action may be related to cell cycle and proliferation, growth factors, metabolic pathways, etc. Different concentrations of CO had different effects, CO granules of various concentrations had no obvious growth-promoting effect (P < 0.05), and so did CO combined with tamoxifen (P > 0.05); high-dose (H-D) CO combined with tamoxifen can induce cell apoptosis (P < 0.05). Through NP, the ACs of CO and related targets of BC were predicted and analyzed. At the same time, it was proved that CO granules are safe in clinical use, and the combined effect of tamoxifen is better in the treatment of estrogen-positive patients, providing reference for the follow-up use of CO in the treatment of BC.