MiR-219a-5p Inhibits Michigan Cancer Foundation-7 (MCF-7) Cell Migration by Regulating Zinc Finger E-Box Binding Homeobox 1 (ZEB1) Expression

Abstract

The potential role of miR-219a-5p in the migration of breast cancer has not been fully elucidated. In this study, bioinformatic analysis revealed that high miR-219a-5p expression in breast cancer tissue was associated with good survival of breast cancer patients. RT-qPCR analysis indicated that miR-219a-5p expression is significantly lower in MDA-MB-231 triple-negative breast cancer (TNBC) cells. In addition, pre-miR-219a overexpression inhibited MCF-7 cell migration and inhibited ZEB1, Twist1 and vimentin expression but promoted the expression of E-cadherin. Moreover, miR-219a-5p mimics inhibited MCF-7 cell migration, whereas MCF-7 cell migration was promoted by the miR-219a-5p inhibitor. Furthermore, miR-219a-5p was found to inhibit the translation of ZEB1 expression by targeting the 5′-ACAAUCA-3′ motif of the ZEB1 3′UTR, and the binding motif is conserved in multiple species. ZEB1 overexpression rescued the inhibition of cell migration induced by miR-219a-5p. Finally, an inverse correlation of miR-219a-5p and ZEB1 expression was observed in four breast cancer cell lines. Thus, miR-219a-5p inhibits MCF-7 cell migration by regulating ZEB1 expression, and ZEB1 is the target gene of miR-219a-5p.