Bone Marrow Mesenchymal Stem Cells (BMSC)-Upregulated miR-139 Inhibited the Migration and Invasion of Breast Cancer Cells In Vitro

Abstract

microRNAs exert a crucial impact on tumor biology. However, the biological effect of miR-139 on breast cancer cells remains unclear yet. Here we intend to clarify the effect and mechanism of miR-139 derived from BMSCs on the biological behavior of gastric cancer cells. Breast cancer cells were divided into BMSC group (mixed culture of BMSC and breast cancer cells 1:1), miR-139 mimics group, si-PXN group and control group followed by analysis of miR-139 level, cell activity by MTT assay and the targeted binding of miR-139 to PXN by luciferase reporter assay. In relative to control, miR-139 level was significantly declined in gastric cancer cells, while PXN level was elevated and associated with the prognosis. miR-139 was up-regulated by BMSCs or miR-139 mimics, thereby regulating EMT process through targeted inhibition of PCN, and ultimately inhibiting the activity of breast cancer cells. In conclusion, BMSC co-culture can inhibit PCN by up-regulating miR-139, thereby regulating EMT process and inhibiting breast cancer progression, implying that miR-139 and PXN could be used as therapeutic targets for metastatic breast cancer.