Src Homology 2 Domain Containing Protein Tyrosine Phosphatase-2 (SHP2) Combined with Dental Pulp Stem Cells Promote the Effect of Angiogenesis

Abstract

Pulpitis is one of the most important dental diseases. How to improve the blood circulation in the infected and necrotic area of pulp is the current research hotspot in the treatment of pulpitis. Mesenchymal stem cells (MSCs) have similar regeneration and differentiation ability to the pluripotent stem cells, and can differentiate into various tissues under certain induced conditions. Dental pulp stem cells (DPSCs) extracted from dental pulp, which have stronger proliferation ability and stability, and are more ideal seed cells for the treatment of pulpitis. Research show that Src homology 2 domain containing SHP2 can promote blood vessel growth. In this subject, we studied the angiogenesis of SHP2 combined with dental pulp stem cells (DPSCs) transplantation. SHP2 and DPSCs were co cultured with human umbilical vein endothelial cells (HUVECs). The proliferation and migration of endothelial cells were detected by Wound Healing Assays. At the same time, the effect of SHP2+DPSC on endothelial cell angiogenesis was examined by tube formation test. The expression of angiogenesis related cytokines including vascular endothelial growth factor (VEGF), von willebrand factor (vWF), Angiopoietin-1 (Ang-1) and Cdc42/Rac1 signal pathway were also detected by Western blot. Our results demonstrated that SHP2 combined with DPSCs can advance endothelial cell angiogenesis. Meanwhile, SHP2+ DPSC obviously increased VEGF, Ang-1 and vWF expression. SHP2+DPSC significantly raise the Cdc42/Rac1 signal pathway in HUVECs. Our data illustrate that SHP2 combined with DPSCs can promote the effect of angiogenesis in pulpitis.