miR-114 Derived from Bone Marrow Mesenchymal Stem Cells Regulates the Metastasis of Prostate Cancer Cells by Targeting P53 Gene

Abstract

Prostate cancer (PCa) in the elderly is a malignancy primary to the prostate and a common tumor in the male urogenital line. However, no effective treatment has been developed for prostate cancer. Previous studies have shown that BMSCs-derived miR-114 can inhibit tumor cell development. Therefore, we intend to determine the role of BMSCs-derived miR-114 and p53 in PCa. Human prostate cancer cells (LNCaP) and BMSCs were inoculated into 12-well plates. After interfering the expression of miR-114 in BMSCs, the culture medium was collected and used to treat LNCaP followed by analysis of cell behaviors. miR-114 inhibited p53 level in BMSC-derived exosomes, thereby inhibiting the proliferation, invasion and migration of PCa cells, and enhancing cell apoptosis. In conclusion, BMSCs-derived miR-114 inhibits the proliferation, migration, invasion and promotes apoptosis of prostate cancer cells by down-regulating p53.