The Effect of LncRNA H19 on Human Dental Pulp Cells Through Tumor Growth Factor-β1 (TGF-β1)/Smad Signaling Pathway

Abstract

The pulp tissue is located in the pulp cavity of tooth and has the ability of nutrition, feeling, and defense. Human dental pulp cells (hDPCs) are the main cells of pulp with the ability to self-renew and multi-directional differentiation. LncRNA H19 is involved in the regulation of human dental pulp stem cells, but the specific mechanism has not been elucidated. hDPCs were isolated and cultured in vitro to measure vimentin expression by immunohistochemistry (IHC). hDPCs were randomly assigned into control group, negative control (NC) group and lncRNA H19 overexpression group followed by measuring lncRNA H19, DSPP, and DMP-1 mRNA expression, cell proliferation, ALP activity, BMP-2 expression by ELISA, TGF-β1, Smad2, and Smad4 expression by Western blot. hDPCs were positive for vimentin staining and confirmed to be derived from mesenchymal. Transfection of pcDNA3.1-LncRNA H19 plasmid significantly increased LncRNA H19 expression, promoted cell proliferation, enhanced ALP activity, upregulated DSPP and DMP-1, elevated BMP-2 expression in cell supernatant, as well as promoted TGF-β1, Smad2, and Smad4 expressions compared with control (P < 0.05). In conclusion, lncRNA H19 facilitates hDPCs differentiation into odontoblasts by promoting cell proliferation and increasing BMP-2 secretion via regulating TGF-β1/Smad signaling pathway.