miR-568 Regulates Bone Marrow Mesenchymal Stem Cells (BMSCs) Homing into Joint Tissue in Rheumatoid Arthritis (RA)

Abstract

This study assessed the biological molecular mechanism of miR-568 on bone marrow mesenchymal stem cells (BMSCs) homing into joint tissue in RA. BMSCs in joint tissue of RA patients was isolated and cultured. The form of BMSCs was observed with microscope and the surface antigen was identified with flow cytometry. The level of miRNA-568, NFATc4, SOX5 and CD17 was detected. The binding relation between SOX5 and miRNA-568 was predicted and validated with multiple kinds of bioinformatics software including Targetscan, miRWalk and miRDB. The level of miRNA-568 in group with BMSCS homing into joint tissue was higher than normal. SOX5 level in RA patients was lower than normal adults. There was a binding relationship between miRNA-568 and SOX5. Overexpression of miR-568 significantly reduced SOX5, NFATc4 and CD17 level. In conclusion, the BMSCs homing into joint tissue could be regulated by miRNA-568 through targeting on SOX5 so as to develop effect on retraining the inflammatory response.