Analysis of the Effects of Curculin on Neuronal Cells, Glutamate Transporter Solute Carrier Family 1, Learning and Memory in Alzheimer’s Disease Rats

Abstract

Our study assessed curculin’s effects on neurons, GLT-1, and behavioral learning ability of AD rats. 40 rats were divided randomly into low curculoside group (CAS1 group), high curculoside group (CAS2 group), normal group (WG group) and model group (GS group) (stereotactic injection of Aβ into hippocampus) followed by analysis of learning ability by Y-maze, GLT-1 level by immunofluorescence histochemistry, neuron morphology by HE staining, GLU content by HPLC, GLT-1 level by western blot, and apoptosis by TUNEL assay. Compared with WG group, GS group had significantly elevated number of exercises and wrong actions, total reaction time and Glu content (20.52±2.09 μmol/L versus 7.51±1.21 μmol/L) and reduced GLT-1 level (0.47±0.06 versus 1.23±0.07) (P < 0.01). In addition, increased apoptosis rate was found in GS group (69.78±9.97) compared to WG group (8.42±2.13) (P < 0.01). Compared with GS group, CAS1 and CAS2 groups had improved changes (P < 0.01) with more improvement in CAS2 group than CAS1 group. In conclusion, curculoside can inhibit GLU expression, reduce neuronal cell apoptosis, and improve the learning ability of AD rats, which may be related to the inhibition of GLT-1 in AD rats.