Astragaloside IV Improve Neurological Function of Cerebral Ischemia

Abstract

This study intends to assess astragaloside IV’s effect on neurological function in mice cerebral ischemia model. The mouse model of cerebral ischemia was established by photochemistry and then assigned into sham operation group (photochemical building do not accept cold light irradiation) and control group (10 ug/ml by intraperitoneal injection of saline solution), drug group (10 ug/ml by intraperitoneal injection of Astragaloside IV) followed by analysis of neurological severity, cerebral infarction area, loss of neurons, glial cell activation and the activities of LC3, Beclin1, Caspase-3, P62 and mTOR by Western Blot. The neurons in cerebral infarction were missing and marginal area and penumbra appeared. The tissue in cerebral infarction became white, and the modeling was successful. The drug group showed significantly reduced scores and decreased infarct area of brain tissue compared with control group on day 14, 21 and 28 (P < 0.05). TUNEL staining showed increased number of TUNEL cells at the ischemic edge in the drug group (0.35±0.07)% (P < 0.05), while the IBAL staining of (27.12±3.01)% and GFAP staining of (0.08±0.02)% in the drug group showed significant inhibition of astrocytes (P < 0.05). The activity of LC3, Beclin1, Caspase-3 and P62 in drug group was inhibited, while the activity of mTOR was promoted. In conclusion, Astragaloside IV improves the balance ability and the neural function of cerebral ischemia repair in mice model.