Research on Mechanism of Nanometer Colloidal Particles of Prostate Specific Membrane Aptamer A10 (PSMA-a10)/TGX221 in Restraining the Transplantation Tumor of Rats with Prostate Cancer (PCa)

Abstract

This study assesses the effect of nanometer colloidal particles of PSMA-a10/TGX22 1 on the transplantation tumor of rats with prostate cancer (PCa). 26 rats with PCa were divided into PPG group, exposed TGX221 group and nanometer TGX221 group followed by analysis of the growth of transplantation tumor, cell apoptotic rate by flow cytometry, and the expression of p110β and Bcl-2 by RT-PCR and Western Blot. Nanometer TGX221 group and exposed TGX221 group showed smaller tumor volume than in PPG group on day 13 after intervention with significant difference of tumor volume between nanometer TGX221 group and exposed TGX221 group on day 7. The inhibiting rate of tumor growth in exposed TGX221 group was less than that in nanometer TGX221 group. However, nanometer TGX221 group presented higher apoptotic rate than other two groups. The level of p110β and Bcl-2 in nanometer TGX221 group was declined gradually with significant differences compared to other two groups. In conclusion, the nanometer colloidal particles of PSMA-a10/TGX221 have promising tumor-targeted specificity possibly through inhibition of Bcl-2 and the activity of p110β, leading to restrained PCa growth.