Magnolol Suppresses Breast Cancer Cells via Regulating miR-140-5p/TLR4 Signaling Pathway

Abstract

Objective: To discuss Mag effects and relative mechanisms in breast cancer treatment by vitro study. Materials and methods: In first step, using difference concentrations of Mag to treat breast cancer cell lines; In next step, the cell liens were divided into NC, Mag and Mag+si-miRNA group. Using MTT to measure cell proliferation rates; using TUNEL and flow cytometry to evaluate apoptosis cell number and rate; measuring invasion cell number and wound healing rate using transwell or wound healing; evaluating relative gene expressions using RT-qPCR and WB assay. Results: Cell proliferation rates, invasion cell number, Ki67 positive cell number, wound healing rates significant depressed (P < 0.05) and cell apoptosis rate and apoptosis cell number significantly increased (P <0.05, respectively), meanwhile, miR-140-5p, TLR4, MyD88 and NF-κB(p65)gene significantly changed (P < 0.05) and TLR4, MyD88 and NF-κB(p65) protein significant down-regulation (P < 0.05). However, with si-miRNA which inhibited miR-140-5p supplement, the cell biological activities significantly increased (P <0.001), with miR-140-5p significant down-regulation, TLR4, MyD88 and NF-κB(p65) significantly up-regulation (P < 0.001). Conclusion: Mag had anti-tumor effects to breast cancer via miR-140-5p/TLR4 axis by vitro cell experiment.