Evaluation of Chemokine (C–X–C motif) Ligand 12 (CXCL12) Function on Homing and Recovering of Stem Cell in Rats with Asherman Syndrome Uterine Injury

Abstract

This study intends to discuss the action of CXCL12 on homing and recovering of stem cell in rats with Asherman Syndrome uterine injury. Forty SD rats were divided into blank group, AS group, CXCL12 group and group of CXCL12 and AMD3100 followed by analysis of the colocalization of cells derived from GFP-positive bone marrow by IF staining, the pathological form of uterus by Masson tricolor and Sirius red staining, as well as the pregnancy rate pregnant time and litter size along with evaluation of the condition of recovery of rats’ uterus. The positive rate of GFP, CD45 and CK in blank group was significantly lower than AS group without significant difference between AS group and AMD3100 group. However, it was significantly higher in CXCL12 group than AS group. There was no abnormal uterus tissue in blank group but notable fibrillation in AS group. The fibrosis level could be reduced in CXCL12 group and AMD3100 group. The pregnancy rate and litter size in blank group was higher than AS group and also higher in CXCL12 group than group of CXC12 with AMD3100. The pregnant time in blank group was less than AS group. The positive expression of GFP, CD45 and CK was upregulated by CXCL12 treatment. In conclusion, the homing and recovering of stem cell in rats with Asherman Syndrome uterine injury are improved and the pregnancy rate and litter size are elevated.