GSK-3β RNAi Lentivirus Affects Neuronal Damage and Nuclear Factor E2-Related Factor 2 (Nrf2) Expression in Cerebral Infarction Rats

Abstract

We investigated the effect of GSK-3β RNAi lentivirus on neuronal damage and Nrf2 level in rats with cerebral infarction. 40 rats were assigned into sham group, CI group, Vector group and GSK-3β RNAi group followed by analysis of cell damage and oxidative stress, neurological scores, cerebral infarction volume, and brain water content as well as brain morphology by H&E staining and Nrf2 protein level by Western blot. Compared with sham group, GSK-3β mRNA in neurons of CI group and Vector group was significantly elevated (P < 0.05) with reduced level in GSK-3β RNAi group (P < 0.05); 3 hours after surgery, there was no change in neuroethology scores of rats in CI group, Vector group and GSK-3β RNAi group (P > 0.05). While 1 and 3 days later, the scores of rats were significantly improved (P < 0.05) and brain water content was reduced in GSK-3β RNAi group (P < 0.05) without difference between CI group and Vector group (P > 0.05). Compared with sham group, infarct size in CI group and Vector group was increased (P < 0.05) and reduced in GSK-3β RNAi group (P < 0.05) without difference between CI group and Vector group (P > 0.05). Meanwhile, CI group and Vector group showed significantly downregulated Nrf2, Srx1 and Trx1 proteins (P < 0.05), which were increased in GSK-3β RNAi group (P < 0.05). In conclusion, GSK-3β RNAi lentivirus can promote the expression of Nrf2 and exert an inhibitory effect on neurons of rats with cerebral infarction, therefore protecting brain tissue.