Bone Marrow Mesenchymal Stem Cells Modified with microRNA-216a-5p Enhance Proliferation of Acinar Cells in Severe Acute Pancreatitis

Abstract

This study assesses the effect of bone marrow mesenchymal stem cells (BMSC) modified with miR-216a-5p on acinar cell proliferation in SAP. 40 rats were equally assigned into miR-NC set, miR-216a-5p set, BMSC set and anti-miR-216a-5p set randomly. The SAP model was prepared using AR42J cells which were disposed with CAE. Cells were transfected with lipidosome method to meaure miR-216-5p by RT-PCR, cell proliferation by CCK-8 along with analysis of cell clone formation and apoptosis. miR-216a-5p in modified BMSC was significantly upregulated compared with BMSC, indicating that BMSC was modified with miR-216a-5p successfully. BMSC modified with miR-216a-5p significantly promoted cell proliferation and clone formation and decreased apoptosis. The luciferase activity in wild type of miR-216a-5p was reduced, indicating that miR-216-5p could target Pak2 gene. In conclusion, proliferation of acinar cells in SAP is prompted and apoptosis ise reduced by BMSC modified with miR-216a-5p, which is possibly through targeting PAK2 gene.