miR-134 Up-Regulates Matrix Metalloproteinase 9 (MMP9) in Chronic Sinusitis

Abstract

Chronic sinusitis is an upper respiratory tract disease. miR-134 involves in several diseases. However, its regulatory mechanism in chronic sinusitis has not been assessed. We aim to explore miR-134’s role in chronic sinusitis and the possible mechanism. miR-134 and MMP9 level was measured in chronic sinusitis tissues and normal tissues. The co-expression of miR-134 and MMP9 in PHNECs was detected by immunofluorescence. MMP-9 expression and IκB and α protein phosphorylation was detected by western blot. Immunofluorescence showed positive MMP-9 expression in epithelial cells. miR-134 level was significantly elevated in patients with chronic sinusitis and was co-localized with MMP-9 in the CRSwNP sample of epithelial cells. miR-134 up-regulated MMP-9, which was inhibited after addition of inhibitor BAY 11-7082. In conclusion, miR-134 up-regulates MMP-9 through NF-κB signaling to mediate the occurrence of chronic sinusitis, indicating that miR-134 may participate in the tissue remodeling of chronic sinusitis.