The Role of RAP1GDS1 in the Inhibition of Glut1 Expression by Catechins in Tongue Squamous Carcinoma Cells

Abstract

Background: The paper aimed to investigate the role of Glut1 in the inhibition of the invasion and migration capabilities of tongue squamous carcinoma cell by Catechin. Materials and Methods: Transwell assay and scratch test were applied to measure the effect of Catechin on the invasion and migration of the cells, and WB to detect the expression of each protein. Overexpressed lentivirus was used to up-regulate Glut1 in tongue squamous carcinoma cells, detecting whether the process can be reversed. After silencing RAP1GDS1 in tongue squamous carcinoma cells by RNAi technology, the effect of Catechin on Glut1 expression was measured. Results: The results of Transwell assay and scratch test verified that Catechin can inhibit the invasion and migration. Subsequent WB experiments confirmed that Catechin can inhibit the expression of Glut1, N-cadherin and Vimentin, while promote the expression of E-cadherin. The up-regulation of Glut1 can significantly reverse the inhibitory effect of Catechin. Silencing of RAP1GDS1 can inhibit the expression of Glut1. However, the up-regulation of Glut1 expression can significantly reverse this process. WB result verified the inhibitive effect of Catechin on RAP1GDS1 in tongue squamous carcinoma cells. Conclusion: Catechin affects glycolytic activity by down-regulating Glut1 and induces EMT process inhibition. It is speculated that, through regulating Glut1 by silencing RAP1GDS1.