Isolation and Culture of Human Bone Marrow Mesenchymal Stem Cells by an Improved Method

Abstract

The harvest of BMSCs has been relying on primary culture because construction of mesenchymal stem cell lines is difficult. The whole bone marrow method is a popular method to isolate and culture BMSCs. But the cell purity and extraction efficiency are not satisfactory using the traditional extraction method. The feasibility of an improved whole bone marrow method for extraction of human BMSCs was explored in our study. After multiple rounds of passage and culture, the cells maintained good morphology and proliferation. The characteristics of human BMSCs were demonstrated via in vitro trilineage-induced differentiation experiment and flow cytometry analysis. In conclusion, human BMSCs can be extracted and cultured successfully and simply using the improved whole bone marrow method.