Exploration of the Potential Mechanism of Succus Bambusae Ameliorating Obesity and Hyperlipidemia in High-Fat Diet Apoe-/-Mice and Caenorhabditis Elegans by Network Pharmacology Combined with Transcriptomic Approach

Abstract

Obesity and hyperlipidemia have become a global public health problem, and bamboo leaves have a positive intervention effect on them. As a common medicinal form of bamboo in clinical practice, the therapeutic effect and mechanism of Succus Bambusae on obesity and hyperlipidemia are still unclear. To this end, two animal models of hyperlipidemia, Apoe-/-mice and Caenorhabditis ele-gans (C. elegans), were used to study the therapeutic effect and potential mechanism of Succus Bambusae on hyperlipidemic by pharmacodynamics, network pharmacology and transcriptomics approachs. The results showed that Succus Bambusae could significantly reduce the lipid accumulation in nematodes, reduce the body weight and liver lipid accumulation in Apoe-/-mice, and significantly reduce the levels of TG and FBG in plasma. Both the arachidonic acid metabolism and the steroid hormone biosynthesis signaling pathways were included in the KEGG pathways enrichment results of network pharmacology and transcriptomics analysis. RT-qPCR results showed that XZL can effectively decrease the transcriptional levels of UGT1A5 and CYP1A1. In summary, XZL can effectively alleviate obesity and hyperlipidemia induced by high-fat diet, which is achieved by regulating the steroid hormone biosynthesis signaling pathways.