Comparative Study of POA-PVS-DBS-GOD Electrode in Acetate and Phosphate Buffers for Determination of Glucose

Abstract

In the present research work, the poly(o-anisidine)-polyvinyl sulphonic acid-dodecylbenzene sulphonic acidglucose oxidase (POA-PVS-DBS-GOD) electrode have been investigated. The silver electrode was used for the synthesis of poly(o-anisidine)-polyvinyl sulphonic acid-dodecylbenzene sulphonic acid (POA-PVS-DBS) composite film using galvanostatic method with 0.2 M o-anisidine, 0.4:0.1 M PVS:DBS, 1.0 pH and 1 mA/cm2 applied current density at room temperature. The synthesized film was characterized by using electrochemical technique, conductivity measurement, UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Glucose oxidase (GOD) was immobilized on synthesized POA-PVS-DBS composite film by cross-linking via glutaraldehyde in acetate buffer and phosphate buffer. The Michaelis-Menten constant (K′m) was determined for the immobilized enzyme. The POA-PVS-DBS-GOD electrode shows the maximum current response at pH 5.5 and potential 0.6 V. The sensitivity of POA-PVS-DBS-GOD electrode in acetate buffer and phosphate buffer has been recorded. The results of this investigation reveal that the phosphate buffer gives faster response as compared to acetate buffer in amperometric measurements.