Preparation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Nanofiber Catheter and Its Mechanism of Nerve Injury in Patients with Cervical Spine Injury

Abstract

At present, surgical suture treatment can be performed for spinal patients after nerve injury, but nerve regeneration and functional recovery require comprehensive treatment including drug treatment. However, there is still a lack of adjuvant therapeutic drugs that can effectively promote nerve regeneration and functional recovery. Drug treatment after nerve injury is the basis of nerve injury treatment and an important supplement to surgical treatment. Finding an effective method for treating spinal nerve injury and studying its mechanism of action may have important basic and clinical significance. The nanofiber catheter material simulates the nano/sub-micron level collagen fiber bundle structure of cells in the body, so it has been more and more widely used in the field of tissue engineering. Therefore, in this study, PHBV nanofiber catheter was successfully prepared by electrostatic spinning method, and the nanofiber catheter was characterized by SEM and DSC tests. The PHBV nanofiber catheter prepared by this research method has excellent characteristics such as porosity, large specific surface area, stable structure, thermal stability, and good mechanical properties. At the same time, adult male SD rats were selected to establish an animal model of cervical spine injury in this experiment. The expressions of three inflammation-related factors (IL-1α, IL-10 and TNF-1) were analyzed by ELISA. The results showed that in the spinal injury group, the expression of the three inflammatory factors all showed a significant increase over time and then reached a peak, then decreased and stabilized. This showed that the PHBV nanofiber catheter repairs cervical spine injury by affecting the inflammatory response, which is conducive to repairing cervical spine injury. RT-PCR was used to detect the expression of CNTF, GAP-43, and Tubulin-related proteins. During the neural regeneration process in rats, the expressions of both backbone proteins continued to be expressed, and they were first up-regulated and then flattened. This indicated that in the early stage of neural regeneration, a large number of skeletal proteins are synthesized, and they continue to be expressed at low levels over time, laying a foundation for the axon skeleton reconstruction.