Synthesis and fluorescence of novel complexes of tricyano-dihydrofuran and ofloxacin

Abstract

Quinolones are widely used, but there are few reports on the application of fluorescent probe analysis. In this paper, we synthesized a tricyano-dihydrofuran (DCDHF-2-V) electron acceptor, and a (DCDHF-2-V)-quinolone fluorescent nanoprobe was designed. Its structure was characterized by infrared spectroscopy (IR), nuclear magnetic resonance (NMR) and ultraviolet (UV). A novel tricyano-dihydrofuran-ofloxacin fluorescent nanoprobe was established for the first time by fluorescence spectrophotometry. A novel fluorescent nanoprobe was obtained and used in the determination of ofloxacin in vitro. The results showed that tricyano-dihydrofuran can form stable charge transfer complex with ofloxacin, and it can be used for the determination of ofloxacin in drugs as a fluorescent probe. While important experimental steps are for the first time achieved in this study for future Quinolone fluorescent nanoprobe implementations, this method is simple and sensitive.