Regulatory Effect of Angelica sinensis Polysaccharide on Bcl-2/Bax/Caspase-3 Signal Pathway in Spleen of Rats with Radiation Injury
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Published:2020-08-01
Issue:4
Volume:14
Page:579-583
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ISSN:1556-6560
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Container-title:Journal of Biobased Materials and Bioenergy
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language:en
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Short-container-title:j biobased mat bioenergy
Author:
Chen Tian-Li,Song Tong-Tong,Sun Xu,Gu Yi-Wen,Du Wen-Jing,Li Xiang
Abstract
To investigate the protective effect of Angelica sinensis polysaccharide (ASP) on spleen injury and its influence of Bcl-2/Bax/Caspase-3 signal pathway in radiation rats, 60 Sprague Dawley (SD) rats were divided into 5 groups randomly: the normal group, the model group, low-, middle-
and high- dose of ASP groups. On the 15th day after the appropriate medication, all the rats except the normal group received the 60Co γ-ray irradiation once with a total dose of 4.0Gy. The levels of NF-kBp65 and IKKa in serum, the contents of MDA, GSH and SOD in spleen of
each group were detected with ELISA after 24 h of irradiation, and the protein expression levels of Bcl-2, Bax, Caspase-3, HSPBP1 and TIMM8B in spleen of each group were detected by western blotting. The results showed that compared with the model group, NF-kBp65, IKKa and MDA contents were
decreased while GSH and SOD contents were increased in the middle and high dose groups. The level of Bcl-2 and TIMM8B were increased while Bax, caspase-3 and HSPBP1 were decreased in all groups. In conclusion, Angelica sinensis polysaccharide can reduce the expression levels of Bax
and caspase-3, while increase the level of Bcl-2 in spleen of rats induced by radiation and antagonize the body injury caused by radiation by regulating Bcl 2/Bax/Caspase 3 signaling pathway.
Publisher
American Scientific Publishers
Subject
Renewable Energy, Sustainability and the Environment,Biomaterials,Bioengineering