Extraction of Damba Black Soybean Gene by Means of Magnetic Nanoparticles and Investigation of Aluminum Tolerance
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Published:2021-04-01
Issue:2
Volume:15
Page:156-162
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ISSN:1556-6560
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Container-title:Journal of Biobased Materials and Bioenergy
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language:en
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Short-container-title:j biobased mat bioenergy
Author:
Lin Tao1,
Han Rongrong1,
Wei Yunmin1,
Liu Lusheng1,
Jiang Caode1,
Yu Yongxiong1
Affiliation:
1. College of Animal Science, Southwest University, Beibei 400700, Chongqing, PR China
Abstract
The aluminum tolerance of Tamba black soybean (Glycine max cv. Tamba) is closely related to organic acid secretion mechanisms. The gene responsible for this tolerance in this variety (GmFER84) is extracted from lysates of soybean root tips using silylated Fe3O4
nanomagnetic beads. GmFER84 (Glycine max XP 003540203.1) is a stable protein. Tobacco genetically transformed with GmFER84 using an Agrobacterium-mediated transformation was tested for aluminum tolerance. Citrate synthase and citric acid secretion in the roots of transgenic tobacco
prove to be significantly higher than those of wild tobacco, and the antioxidant properties of transgenic tobacco are also substantially increased. Research on GmFER84 may enable further agronomic development.
Publisher
American Scientific Publishers
Subject
Renewable Energy, Sustainability and the Environment,Biomaterials,Bioengineering