Quantitative Trait Loci Mapping of Grain Lipid Content in Rice Variety ‘Goudang 3’ Using Genome Re-Sequencing

Abstract

Fat is the third largest substance in rice seeds, and its content has a great impact on rice cooking, taste and storage qualities. However, few genes related to seed fat content have been cloned, which seriously restricts the application of fat traits in high-quality rice breeding. ‘Goudang 3’ is a representative aromatic rice landrace of Guizhou He, a characteristic rice species resource in Guizhou Province, China. It has the advantages of high fat content, rich flavor, soft and glutinous taste, etc. In this work, to address this issue, from a high-fat content rice variety, Goudang 3, and a low-fat content rice variety, Huazhan, a 148 recombinant inbred lines (RILs) population were derived. A high-density genetic map with 4063 Bin Markers covering 12 chromosomes were constructed through simplified genotyping-by-sequencing (GBS) technology. In combination with fat content, a brown rice fat content quantitative trait loci (QTL) qCFC3 was mapped within the 492 kb interval on chromosome 3. The qCFC3 was consistent with the previous reporting site, and no cloned fat content-related genes have been reported. LOC_Os03g13630 was identified. In conclusion, these QTL and candidate gene can be used for improving rice quality and can lay a foundation for further cloning and functional analysis.