An Enzyme-Linked Immunosorbent Assay for C-Reactive Protein Based on Gold Nanoparticles@Metal Porphyrin Porous Compound

Author:

Wang Min,Meng Shuang,Gu MengQiao,Chi KuanNeng,Ma YuChan,Deng Yan,Zhang Xi,Li Delei,Hu Rong,Yang Yunhui

Abstract

In this paper, an enzyme-linked immunosorbent assay (ELISA) using gold nanoparticles/metal porphyrin porous compound (Au NPs@PAF-40-Fe) as a marker was developed. Quantitative detection of C-reactive protein (CRP) was achieved by UV-visible spectrophotometry under optimal conditions. The sensor has a good linear relationship in the range of 0.05–2000 ng/mL. The lower limit of detection was 0.017 ng/mL (S/N = 3), the linear correlation coefficient was 0.9921, and the average recovery was 100.74%. The ELISA has good selectivity and provides a sensitive way for detection of CRP.

Publisher

American Scientific Publishers

Subject

Pharmaceutical Science,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

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