Author:
Yang Tong,Wu Chao,Li Zhiyang,Chen Yuxin,Chen Hui,Gao Hansong,Liu Mei,He Nongyue,Liu Hongna,Liu Bin,Chen Zhu,Elingarami Sauli,Shah Muhammad Ali
Abstract
With the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. A device has been developed for real-time monitoring the process of loop-mediated
isothermal amplification reaction through photoelectric detection technology and temperature control technology. This paper aimed at the designing protocol and the application of real-time turbidity detection system by LAMP by means of that magnesium ions can combine pyrophosphate ions by-product
of LAMP reaction to form a white precipitate of magnesium pyrophosphate. The change of sample turbidity can be detected by a photoelectric sensor. The system is easy and friendly to operate and the amplification process of 8 samples can be completed simultaneously at 63 °C in one hour.
In the end, the experiment results suggest that the real-time turbidity detection system is stable and reliable.
Publisher
American Scientific Publishers
Subject
General Materials Science
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献