Protective Effect of Hydroxysafflor Yellow A on Isoproterenol-Induced Myocardial Fibrosis in Rats

Abstract

Aim: Present research was to explore the effects and mechanisms of hydroxysafflor yellow A in treatment of isoproterenol-induced myocardial fibrosis in rats. Methods: Dividing 50 SD rats as the normal control, ISO and HSYA treated groups, 10 rats in each group. Administering Rats by ISO subcutaneously (2 mg/kg · d) for 10 days induce myocardial fibrosis. HSYA treated groups were treated with 10, 20 and 40 mg/kg · d HSYA for 4 weeks (ig). The normal control and the model groups were treated with saline at equivalent volume. Measurement of hemodynamics index to evaluate the changes of cardiac function. And evaluating the relative index of difference groups. Results: Treatment of HSYA significantly inhibited heart rate (HR) and LVEDP, meanwhile improving LVSP compared with model group with dose-dependent. HE staining and Masson staining showed that diltiazem improve heart structure disorder, excessive accumulation of collagen fibers, meanwhile decreasing the value of collagen volume fraction (CVF) (P < 0.05). The result showed diltiazem inhibited Collagen I and Collagen II which were up-regulation (P < 0.001). HSYA could significantly increase SOD, GSH-Px levels (P < 0.001), meanwhile reducing the MDA levels in ISO-induced myocardial fibrosis model (P < 0.001) with dose-dependent. In addition, the elevated NF-κB, TGF-β1, CTGF expressions were effectively restored by HSYA(P < 0.001). Conclusion: HSYA alleviated ISO induced myocardial fibrosis in rat, which might be correlation with the improvement of reduction of oxidative stress and inhibition of NF-κB signal pathway.