Effects of Fluorouracil-Carrying Nano Targeted Liposomes on Proliferation, Migration, and Invasion of Gastric Cancer Cells

Abstract

Fluorouracil (FU) is a common chemotherapy drug. To overcome the shortcomings of the original drug, such as strong gastrointestinal reaction and short half-life, FU was placed in the Mahadevi layered complex (MLC) added with dextran (DEX) for surface modification to obtain the magnetic targeting nanoparticles (MLC-Dex-Fu) with sustained release. Next, the MLC-Dex-Fu was combined with liposome to obtain the FU-carrying nano targeted liposome (LMDF). MGC-803 gastric cancer (GC) cells were selected to establish the nude mouse model of GC solid tumor. The nude mice were rolled into different groups (a control group (Ctrl group), a FU group, a LMDF group without magnetic field, and a LMDF group with magnetic field). The diet and weight of nude mice were observed after interventions under the conditions of NMF and MF. The tumor tissues were removed, cleaned, cut into homogenate, transferred to the pre-cooled Eppendorf (EP) tube, and then performed with homogenation, suction by pipettes, and filtration to obtain the tumor cell suspensions of the corresponding groups. Partial tumor cell suspensions were based to analyze the cell proliferation by flow cytometry (FCT), and the other suspensions were based to investigate the migration and invasion by the Transwell method. The results suggested the cell viability in the LMDF groups was greatly decreased (P < 0.05). In the nude mouse model test, LMDF exhibited a good magnetic targeted transport and sustained release chemotherapy, and could guide FU to move in vivo and enrich in the tumor site with a high concentration under the effects of external magnetic field, thus inhibiting the tumor proliferation. Such efficacy was more obvious in comparison with other groups (P < 0.05).