miR-212-3p Promotes Synovial Inflammation in Osteoarthritis by Regulating 11β-Hydroxysteroid Dehydrogenase 1/Glucocorticoid/Glucocorticoid Induced Leucine Zipper Signaling Axis in Human Synovial Fibroblasts

Abstract

To investigate the regulatory effect and mechanism of miR-212-3p-mediated 11β-HSD1/GC/GILZ signal axis on the inflammatory response of human synovial fibroblasts (HFLS). HFLS cells were cultured and divided into Control group, inhibitor-NC group and miR-212-3p group. Cell activity, apoptosis, inflammatory factors and 11β-HSD1/GC/GILZ signal axis expression were observed in each group. After inhibiting the expression of miR-212-3p, the HFLS cell inflammation model constructed by IL-1β increased cell activity, decreased apoptosis rate, decreased expression of inflammatory factors, and increased expression of 11β-HSD1, cortisol and GILZ. In conclusion, miR-212-3p targeted down-regulated the expression of 11β-HSD1. Inhibition of miR-212-3p can regulate the 11β-HSD1/GC/GILZ signaling axis to promote the reversal of IL-1β-mediated HFLS cell inflammatory response.