Protective Effect of Klotho on Acute Kidney Injury Caused by Contrast Agents

Abstract

Contrast-induced nephropathy (CIN) is currently the third leading cause of iatrogenic renal failure. This research mainly explores the protective effect of Klotho on CIN. Klotho (10 ng/g) was administered after the establishment of a CIN mouse model, and the changes of tissue structure and renal function were observed, and the Wnt/β-catenin pathway and senescence-related factors were detected. Compared with the control group, the brush border of renal tubular epithelial cells disappeared, cytoplasmic vacuole-like changes, renal tubular dilatation, and renal function were significantly reduced in the model group. Klotho treatment can effectively alleviate changes in renal tubular epithelial structure and function. Compared with the model group, Klotho treatment can obviously inhibit the expression of P27 and P53, and promote the expression of Sirt1 and Bmi-1, and Klotho can regulate the Wnt/β-catenin pathway. Exogenous Klotho can slow down nephrocyte senescence by regulating the Wnt/β-catenin pathway, thereby protecting kidney.