The Mechanism of ITGB3 and PPIF Plasmid Construction on the Offspring of Rats with Intrauterine Adhesion and Bioinformatics Analysis

Abstract

This study aimed to develop an animal model of pathologic regeneration of intrauterine adhesions (IUA) and assess the impact of ITGB3 and PPIF plasmid construction on the offspring of rats with IUA. Thirty female SD rats were divided into an experimental group and a control group. In the experimental group, the rats’ left and right endometrium underwent mild and severe mechanical damage using a self-made curette. The control group underwent a sham operation without endometrial injury. At various time points after surgery, uteruses were collected for analysis. Immunohistochemical staining was performed to evaluate changes in major histocompatibility complex II molecule (MHC II) and 5-bromodeoxyuridine (BrdU) in endometrial cells. The study observed the inflammatory and hyperplastic changes in endometrial tissue and the repair process in rats with different endometrial gland injuries. RNA interference (RNAi) sequences targeting the rat PPIF gene were designed and cloned into a lentivirus vector transfer plasmid, ITGB3. The number of endometrial glands decreased with increased mechanical injury. The PPIF short hairpin RNA (shRNA) fragment was successfully cloned into the lentiviral vector. After mild mechanical injury, the endometrial tissue regenerated to a basic repair level, while severe mechanical injury led to incomplete repair and tissue fibrosis, resulting in IUA.