Construction and performance study of double-layer periosteal material for repairing bone tissue damage

Abstract

Microscopic observation is necessary for the treatment of bone tissue microloss. To observe the process of bone tissue maturation is an essential research content in the field of orthopaedics. In many cases, people have to distinguish between new bone and mature bone, sometimes involving software calculation, and conventional HE staining obviously cannot fully meet the requirements. The quality of imaging directly affects the therapeutic effect of medical staff. In order to prove the effect of laser scanning confocal microscope (LSCM) on the observation of bone tissue micro damage, a piece of pig iliac bone and a piece of human femoral head were selected. The two kinds of bone tissue were divided into three small pieces respectively, and then stained with calcein green and alizarin red, or added with 5 ml of 1000 ml/L ethanol, and then placed under the LSCM and fluorescein Scanning under light microscope. This article uses silk fibroin as the raw material to prepare high-strength bilayer membranes using rotary evaporation method. Through relevant performance experiments, the results show that the mechanical properties of the double-layer silk fibroin film are about 12.48 MPa, and the tensile strength at break is about 13.66%. With the addition of hydroxyapatite, the mechanical properties of the silk fibroin film are improved. The addition of VEGF has little effect on the mechanical properties, and the addition of growth factor has little effect on the tensile strength of the material. The double-layer silk fibroin membrane was degraded in protease for 2 weeks, with a mass loss of about 19.3%. There was almost no degradation phenomenon in PBS solution; The release of VEGF from the double-layer silk fibroin membrane was about 17.3% after 2 weeks, with a relatively fast release rate in the early stage, and then the rate slowed down. The total release of VEGF decreased compared to the pure SF/VEGF membrane. Through the analysis of the scanning image, it is found that the image signal-to-noise ratio of alizarin red stained human femoral head is 6.956 higher than that of fluorescence microscope. In terms of image error rate, fluorescence microscope is 4.228 higher than laser scanning confocal microscope. The edge definition of laser scanning confocal microscope was 22.17 higher than that of fluorescence microscope, and the image clarity of stained bone tissue was higher than that of bone tissue only added ethanol. Therefore, the imaging effect of bone tissue staining is better, and the image generated by LSCM is higher than that generated by fluorescence microscope.