Mechanism of polyethylene glycol and polyethylenimine combined with superparamagnetic nanoparticles in treating the brain glioma

Abstract

This study assessed the mechanism of polyethylene glycol (PEG) and polyethylenimine (PEI) combined with superparamagnetic nanoparticles in treating brain glioma. Forty-eight male Stromal Derived Factor (SDF) grade Sprague Dawley (SD) rats were divided into control set, doxorubicin (DOX) set, set of DOX and Spions Medicine (SPIONs) and set of DOX, SPIONs and magnet followed by analysis of change of tumor volume, MRI imaging, HE staining, trypan-blue staining, and TUNEL staining. The dispersion of DOX-SPIONs nanoparticles was uniform. The form was circular. The average diameter was 15.4 nm. The saturation magnetization value of SPIONs and DOX-SPIONs were 45.8 emu · g and 23.6 emu · g. There was a good superparamagnetism in prepared magnetic nanoparticles. The tumor volume in set of DOX, SPIONs and magnet was the smallest. It was smaller in set of DOX and SPIONs than in DOX set and control set. There were excessive cells in tumor tissue in control set, DOX set and set of DOX and SPIONs. There were notable multifarious nucleuses. The quantity of tumor cells in set of DOX, SPIONs and magnet could be reduced. There were notable apoptosis and necrosis. The growth of brain glioma could be restrained by PEG and PEI combined with superparamagnetic nanoparticles through targeting DOX. The apoptosis could be prompted. The superparamagnetic nanoparticles could be prompted to be gathered in tumor site. The therapeutic effect of DOX could be improved through DOX carried with PEG and PEI combined with superparamagnetic nanoparticles. There are promising therapeutic values in the treatment with superparamagnetic nanoparticles through targeting DOX on brain glioma.