miR-15a nanometric compound restrains the activity and adhesion of Hela cells through survivin signal pathway

Abstract

We herein studied the mechanism of miR-15a nanometer compound (NMC) by assessing its activity and adhesion in Hela cells through survivin signal pathway. The miR-15a nanometer compound was prepared and Hela-S3 cells were divided into miR-15a group, miR-NC group and NC group for in vitro studies. Proliferative activity was detected with MTT method while invasive ability was detected with Transwell closet method. Adhesive capacity was compared and Survivin and Caspase-3 protein expressions were detected with Western blot assay. Results showed that, grain size of miR-15a NMC was reduced along with increased N/P value, and Zeta potential was negative, and was converted into positive charge along with increased N/P value. The particle size distribution was uniform and potential was moderated when the N/P value was twenty. The proliferation ability and invasive capacity in miR-15a group was reduced significantly along with lower A540 value and higher adhesive ability as well as reduced survivin and increased Caspase-3 level. The proliferative, invasive and adhesive capacity of Hela cells were all restrained by the miR-15a NMC, and apoptosis was prompted through restraining of the survivin signaling pathway, so as to affect the occurrence and development of cervical carcinoma.