Antioxidant, cytotoxicity, and anti-prostate carcinoma effects of CuO nanoparticles containing Thymus vulgaris

Abstract

In this work, we have described the green supported of CuO NPs over Thymus vulgaris (CuNPs) as a reducing/stabilizing nanocomposite in alkaline medium. In the cellular and molecular part of the recent study, the treated cells with CuNPs were assessed to determine the cytotoxicity and anti-human prostate carcinoma properties on prostate carcinoma cell lines i.e., LNCaP clone FGC-Luc2, 22Rv1, and NCI-H660. The morphological and physicochemical features of the prepared nanocomposite were determined using several advanced techniques as Field Emission Scanning Electron Microscopes (FE-SEM), Ultraviolet–visible spectroscopy (UV-Vis), and fourier transform infrared spectroscopy (FTIR) studies. In the antioxidant test, the IC50 of CuNPs and butylated hydroxytoluene (BHT) against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals were 94 and 88 μg/mL, respectively. The IC50 of CuNPs were 191, 275, and 250 μg/mL against LNCaP clone FGC-Luc2, 22Rv1, and NCI-H660 cell lines, respectively. In conclusion, our data suggest that the malignant prostate cell lines viability decreased in the CuNPs presence.