A test study of different nanofibre scaffolds in tracheal cartilage repair

Abstract

Tracheal abnormalities occur in situations such as tracheal stenosis and bronchitis, causing serious adverse effects on the normal life of patients. Tracheal cartilage is one of the important tissues that maintain airway patency, but it is difficult to repair by itself. The electrospinning technology can be combined with growth factors, which has a certain positive effect on the repair of cartilage tissue. The study utilized coaxial electrospinning technology to prepare nanofiber scaffolds loaded with Kartogenin, and analyzed the effects of different nanofiber scaffolds on tracheal cartilage repair through experiments such as inducing bone marrow mesenchymal stem cell differentiation, in vitro release, and in vitro degradation. The results showed that within 87 seconds, the contact angle of the nanofiber scaffold corresponding to Kartogenin decreased from 121.94° to 50.14°. Kartogenin of 10±3 μM does not cause cytotoxicity to chondrocytes after 7 days of cultivation, and can maintain its phenotype well. As the transcription level of chondrocyte marker genes gradually increases, Kartogenin can induce bone marrow mesenchymal stem cells to differentiate into chondrocytes. The above results verify the good mechanical properties of Kartogenin after successfully loading into nanofibers, as well as its good cell compatibility, promoting chondrocyte differentiation, and providing a new method reference for tracheal cartilage repair.